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Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.


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Product Image-786-249

Protein fractionation kit, FOCUS™ Membrane Proteins

Catalogus nummer: (786-249)
Leverancier: G-Biosciences
Omschrijving: FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.
UOM: 1 * 1 Kit
Product Image-786-257

FOCUS™ Yeast proteome kits

Catalogus nummer: (786-257)
Leverancier: G-Biosciences
Omschrijving: Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM: 1 * 1 Kit
Product Image-786-244

SilverOUT™, G-Biosciences

Catalogus nummer: (786-244)
Leverancier: G-Biosciences
Omschrijving: SilverOUT removes interfering silver ions from gel pieces.
UOM: 1 * 1 EA
Product Image-786-681

InGel™ Blue for protein digestion

Catalogus nummer: (786-681)
Leverancier: G-Biosciences
Omschrijving: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.
UOM: 1 * 1 Kit
Product Image-786-277

Antibody Fab and F(ab)₂ fragmentation of mouse IgG₁ kits

Leverancier: G-Biosciences
Omschrijving: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG<sub>1</sub> Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Image Unavailable-ENZOADI9502710500

Antigen retrieval reagent, IHC

Leverancier: ENZO LIFE SCIENCES
Omschrijving: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

New Product

Product Image-786-022

Protein fractionation kit, FOCUS™ mitochondria

Catalogus nummer: (786-022)
Leverancier: G-Biosciences
Omschrijving: FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.
UOM: 1 * 1 Kit
Product Image-PIER90056

Endoproteinase chymotrypsin protease (TLCK treated), MS grade, Pierce™

Catalogus nummer: (PIER90056)
Leverancier: Thermo Fisher Scientific
Omschrijving: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
UOM: 1 * 1 EA
Product Image-PIER90053

Endoproteinase Asp-N protease, MS grade, Pierce™

Catalogus nummer: (PIER90053)
Leverancier: Thermo Fisher Scientific
Omschrijving: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 1 EA

MSDS


Product Image-PIER90054

Endoproteinase Glu-C protease, MS grade, Pierce™

Catalogus nummer: (PIER90054)
Leverancier: Thermo Fisher Scientific
Omschrijving: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 1 EA

MSDS


Product Image-786-599

Immobilised Iminobiotin Resin

Catalogus nummer: (786-599)
Leverancier: G-Biosciences
Omschrijving: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.
UOM: 1 * 5 mL
Product Image-786-258

Proteome kits, FOCUS™ Bacterial Proteome

Catalogus nummer: (786-258)
Leverancier: G-Biosciences
Omschrijving: Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from <i>E.coli</i>, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM: 1 * 1 Kit
Product Image-786-251

Albumin removal kit, AlbuminOUT™

Leverancier: G-Biosciences
Omschrijving: Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.

Image Unavailable-786-214

Detergent removal system, DetergentOUT™ Tween®

Leverancier: G-Biosciences
Omschrijving: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

Certificaten

Product Image-ENZOENZ450140100

EpiXtract® total histone extraction kit

Catalogus nummer: (ENZOENZ450140100)
Leverancier: ENZO LIFE SCIENCES
Omschrijving: Rapid, reliable method to isolate histone proteins in mammalian cells and tissues for histone modification applications.
UOM: 1 * 1 Kit

New Product


Product Image-786-247

Protein fractionation kit, FOCUS™ soluble and insoluble

Catalogus nummer: (786-247)
Leverancier: G-Biosciences
Omschrijving: FOCUS™ Soluble & Insoluble is a complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilise difficult proteins.
UOM: 1 * 1 Kit
Bel voor prijs
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
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