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Enzyme-linked immunosorbent assays identify and calculate substances such as proteins, peptides, antibodies, and hormones. The provided reagents and supplies allow personnel to combine an immobilized antigen with an antibody that is joined to an enzyme. Following with substrate incubation, the produced antigen-antibody interaction and following conjugated enzyme activity can be measured and assessed. The ELISA kits are available for classroom simulations as well as for professional laboratory purposes.