U heeft gezocht op: Koppelings- en labelingssystemen voor eiwitten

Leverancier: Biotium

Omschrijving: Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).

MSDS

Leverancier: Biotium

Omschrijving: CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labelled DNA probes for <i>in situ</i> hybridisation and nucleic acid blotting applications.

Catalogus nummer: (92224.)

Leverancier: Biotium

Omschrijving: This kit contains everything needed to label antibodies or other proteins with biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labelling reactions of up to 1 mg protein per reaction.

UOM: 1 * 1 Kit

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Catalogus nummer: (ENZOENZ42831L0050)

Leverancier: ENZO LIFE SCIENCES

Omschrijving: Green 496 dUTP (lyophilised)

UOM: 1 * 1 EA

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Catalogus nummer: (ENZOENZ42831)

Leverancier: ENZO LIFE SCIENCES

Omschrijving: Green 496 [5-Fluorescein] dUTP can replace TTP in reactions in which it serves as a substrate for <i>E. coli</i> DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

UOM: 1 * 1 EA

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Catalogus nummer: (ENZOENZ42853L0050)

Leverancier: ENZO LIFE SCIENCES

Omschrijving: Aqua 431 dUTP (lyophilised)

UOM: 1 * 1 EA

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Catalogus nummer: (ENZOENZ53007C200)

Leverancier: ENZO LIFE SCIENCES

Omschrijving: Mixture of fluorescent dyes for detection of lysosomes, mitochondria and nucleic acids

UOM: 1 * 200 µl

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Catalogus nummer: (ENZOALX620076M001)

Leverancier: ENZO LIFE SCIENCES

Omschrijving: Fluorescent probe for Zn²⁺. Cell permeable.

UOM: 1 * 1 mg

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Catalogus nummer: (ENZOALX850023KI01)

Leverancier: ENZO LIFE SCIENCES

Omschrijving: The kit offers a set of six different MAbs specific for serine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries. Clone 16B4 strongly interacts with pSK and pSP motifs, thus specifically recognizes substrates of MAP/SAP Kinases and CDC kinases.Clones 1C8, 4A3, 4A9, 4H4 and 7F12 interact with epitopes containing phosphoserine in the context of positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.) Though revealing a similar pattern in epitope mapping using phosphopeptide libraries, these antibodies interact with different sets of phosphoproteins in whole cell lysates.

UOM: 1 * 1 EA

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Leverancier: Biotium

Omschrijving: ExoBrite™ CTB EV Staining Kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ CTB EV Stains bind to molecules in the EV membrane for bright, specific staining, with little to no background.

Leverancier: Biotium

Omschrijving: Reactive quenchers that act as acceptors in fluorescence resonance energy transfer (FRET) applications.

Leverancier: Cayman Chemical

Omschrijving: Bilirubin conjugate is a stable, water-soluble ditaurate derivative of bilirubin meant to mimic endogenous bilirubin glucuronide derivatives.

Catalogus nummer: (APOSBIL3113-100ML)

Leverancier: Apollo Scientific

Omschrijving: Liquid product red odourless pH 6,7 to 6,9. Protein free Stabiliser. Enzyme activity preservation by special chemistry.

UOM: 1 * 100 mL

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Leverancier: Biotium

Omschrijving: The simplicity and convenience of Mix-n-Stain™ labelling, for conjugation of 1 mg IgG with fluorescent CF® dyes or other dyes.

Leverancier: G-Biosciences

Omschrijving: The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.

Catalogus nummer: (COBSD4-000)

Leverancier: Columbia Biosciences

Omschrijving: Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore.

UOM: 1 * 1 mg

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