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Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Product Image-1B1301-KIT

VWR® Protein Precipitation Kit

Omschrijving: The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.
Catalogus nummer: 1B1301-KIT
UOM: 1 * 1 Kit
Leverancier: VWR Chemicals
Image Unavailable-D8284-5G

Bicinchoninic acid disodium salt hydrate

Omschrijving: Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.
Catalogus nummer: D8284-5G
UOM: 1 * 5 g
Leverancier: SIGMA ALDRICH MICROSCOPY
Image Unavailable-33022-T

Thermal shift protein stability kits, GloMelt™

Omschrijving: Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.
Catalogus nummer: 33022-T
UOM: 1 * 1 Kit
Leverancier: Biotium

MSDS


Image Unavailable-CAYM10005020-96

Protein carbonyl colorimetric assay kit

Omschrijving: The Protein Carbonyl Colorimetric Assay Kit is for the measurement of oxidised proteins. Protein samples are derivatised by making use of the reaction between 2,4-dinitrophenylhydrazine and protein carbonyls.
Catalogus nummer: CAYM10005020-96
UOM: 1 * 1 EA
Leverancier: Cayman Chemical
Product Image-786-573

Bicinchoninic acid (BCA) reducing agent compatible protein Assay

Omschrijving: The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP.
Catalogus nummer: 786-573
UOM: 1 * 1 EA
Leverancier: G-Biosciences
Image Unavailable-TNB-1213-L150

Foxp3 / Transcription Factor Staining Buffer Kit, Tonbo Biosciences

Omschrijving: Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry
Catalogus nummer: TNB-1213-L150
UOM: 1 * 150 mL
Leverancier: Tonbo Biosciences
Product Image-786-603

Protein assay kits, RED 660™

Omschrijving: RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms many commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays. It offers greater linearity, colour stability, and compatibility with detergents, reducing agents and other interfering agents compared to the Coomassie assays. The single, ready to use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.
Catalogus nummer: 786-603
UOM: 1 * 10 Flacon
Leverancier: G-Biosciences
Product Image-ROCKMB-061-0100

Conjugate stabilisers

Omschrijving: Conjugate stabilisers are used with horseradish peroxidase (HRP) and calf intestinal alkaline phosphatase conjugated primary or secondary antibodies or other proteins or compounds in solution. The components of the stabiliser (proprietary) are unique and stabilise both the antibody and enzyme portions of the conjugate.
Catalogus nummer: ROCKMB-061-0100
UOM: 1 * 100 mL
Leverancier: Rockland Immunochemicals
Image Unavailable-ENZOENZ51029K100

eFluxx-ID® multidrug resistance assay kit

Omschrijving: The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP. The assay uses a hydrophobic, non-fluorescent compound that readily penetrates the cell membrane, where it is hydrolyzed to a hydrophilic fluorescent dye by intracellular esterases.
Catalogus nummer: ENZOENZ51029K100
UOM: 1 * 1 Kit
Leverancier: ENZO LIFE SCIENCES
Product Image-CAYM700480-96

Glycogen Assay Kit

Omschrijving: A simple and sensitive tool for assaying glycogen from tissue.
Catalogus nummer: CAYM700480-96
UOM: 1 * 1 ST
Leverancier: Cayman Chemical
Product Image-ENZOENZ51023KP050

PROTEOSTAT® protein aggregation assay

Omschrijving: PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures.
Catalogus nummer: ENZOENZ51023KP050
UOM: 1 * 1 EA
Leverancier: ENZO LIFE SCIENCES
Product Image-786-005

Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay

Omschrijving: A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and others.
Catalogus nummer: 786-005
UOM: 1 * 1 Kit
Leverancier: G-Biosciences
Image Unavailable-ENZOBMLUW1000A0001

SUMO-QAPTURE-T® kit for isolation and enrichment of SUMOylated proteins

Omschrijving: The only commercially available kit for isolation and enrichment of SUMOylated proteins.
Catalogus nummer: ENZOBMLUW1000A0001
UOM: 1 * 1 EA
Leverancier: ENZO LIFE SCIENCES
Product Image-CAYM700510-96

L-Lactate Assay Kit

Omschrijving: A fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva.
Catalogus nummer: CAYM700510-96
UOM: 1 * 1 ST
Leverancier: Cayman Chemical
Image Unavailable-GENO786-1084

Compatible Lowry protein assay

Omschrijving: These CL (Compatible Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The CL Protein Assay improves upon the traditional Lowry method to be compatible with common laboratory agents known to interfere with Lowry protein assays such as reducing agents (dithiothreitol (DTT), ß-mercaptoethanol and TCEP), detergents, chelating agents, amines, sugars, strong chaotropic buffers, salts, drugs, antibiotics, cobalt and other common laboratory agents.
Catalogus nummer: GENO786-1084
UOM: 1 * 1 Kit
Leverancier: G-Biosciences
Product Image-786-571

Bicinchoninic acid (BCA) protein assay

Omschrijving: The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions.
Catalogus nummer: 786-571
UOM: 1 * 1 Kit
Leverancier: G-Biosciences

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