Supelco® Ascentis® RP-Amide HPLC Columns

Leverancier: Merck
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Supelco® Ascentis®
565320-UEA 569 EUR
565320-U 565344-U 565322-U 565321-U 565300-U 565305-U 565328-U 565370-U 565306-U 565371-U 565327-U 565372-U 565373-U 565347-U 565301-U 565313-U 565324-U 565348-U 565323-U 565302-U 565312-U 565303-U 565325-U 565304-U
Supelco® Ascentis® RP-Amide HPLC Columns
Kolommen voor chromatografie
Ascentis® RP-Amide HPLC columns are optimised to the three terms of the resolution equation: efficiency, retention, and selectivity.

  • High purity, type B silica for inertness, reproducibility, and stability
  • Modern bonding process optimises bonded phase coverage and maximises stability, while minimizing bleed and unwanted secondary interactions
  • Compatible with LC-MS and all of today's sensitive instruments and methods
  • Scalable sensitivity from analytical to preparative
  • High surface area silica for high preparative loading capacity
  • Impurity: <5 ppm metals
  • Surface coverage: 2,7 µmol/m²

Ascentis® RP-Amide can be used for many of the same separations as a C18 while avoiding some of the disadvantage of C18, such a poor wettability in high aqueous mobile phases. In addition, it is much more retentive for those molecules that can interact by hydrophobic interactions and also by H-bonding with the amide group. Compared to alkyl only phases, Ascentis® has enhanced retention and selectivity for phenols, organic acids, and other polar solutes due to strong H-bonding between the amide carbonyl (H-bond acceptor) and H-bond donors, like phenols and acids. Compared to other embedded polar group (EPG) phases, like carbamates, ureas, sulphonamides and ethers, Ascentis® RP-Amide gives retention comparable to C18 and C8 for easy column comparison without the need to change mobile phase conditions.

Ascentis® bonded phases have a wide range of selectivities. It is likely that one or more Ascentis® phase will accomplish any small molecule HPLC separation. Packed in micro- to preparative hardware dimensions, Ascentis® products cover all HPLC application areas, including the most sensitive trace-level analyses.

Certificaten: Column test certificate available inside the box.
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