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U heeft gezocht op: Reagentia voor eiwitextractie

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Product Image-DGA01

Optimizer blueBALLS™

Catalogus nummer: (DGA01)
Leverancier: G-Biosciences
Omschrijving: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.
UOM: 1 * 500 ST
Product Image-786-793

Immobilised Ficin

Catalogus nummer: (786-793)
Leverancier: G-Biosciences
Omschrijving: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.
UOM: 1 * 5 mL
Product Image-786-598

Biotin resin

Catalogus nummer: (786-598)
Leverancier: G-Biosciences
Omschrijving: Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.
UOM: 1 * 5 mL
Product Image-786-244

SilverOUT™, G-Biosciences

Catalogus nummer: (786-244)
Leverancier: G-Biosciences
Omschrijving: SilverOUT removes interfering silver ions from gel pieces.
UOM: 1 * 1 EA
Product Image-786-681

InGel™ Blue for protein digestion

Catalogus nummer: (786-681)
Leverancier: G-Biosciences
Omschrijving: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.
UOM: 1 * 1 Kit
Image Unavailable-ENZOADI9502710500

Antigen retrieval reagent, IHC

Leverancier: ENZO LIFE SCIENCES
Omschrijving: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

New Product

Product Image-786-241

InGel™ for protein digestion

Catalogus nummer: (786-241)
Leverancier: G-Biosciences
Omschrijving: InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.
UOM: 1 * 1 Kit
Product Image-786-077

Protein reducing agents

Leverancier: G-Biosciences
Omschrijving: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Image Unavailable-ENZOBMLKI1761250

Fluor-de-Lys® Developer II concentrate

Catalogus nummer: (ENZOBMLKI1761250)
Leverancier: ENZO LIFE SCIENCES
Omschrijving: FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
UOM: 1 * 1,25 mL

New Product


Product Image-PIER88701

Universal nuclease for cell lysis, Pierce™

Leverancier: Thermo Fisher Scientific
Omschrijving: The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.

Product Image-PIER90054

Endoproteinase Glu-C protease, MS grade, Pierce™

Catalogus nummer: (PIER90054)
Leverancier: Thermo Fisher Scientific
Omschrijving: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 1 EA

MSDS


Product Image-PIER90056

Endoproteinase chymotrypsin protease (TLCK treated), MS grade, Pierce™

Catalogus nummer: (PIER90056)
Leverancier: Thermo Fisher Scientific
Omschrijving: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
UOM: 1 * 1 EA
Product Image-PIER90053

Endoproteinase Asp-N protease, MS grade, Pierce™

Catalogus nummer: (PIER90053)
Leverancier: Thermo Fisher Scientific
Omschrijving: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 1 EA

MSDS


Product Image-786-599

Immobilised Iminobiotin Resin

Catalogus nummer: (786-599)
Leverancier: G-Biosciences
Omschrijving: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.
UOM: 1 * 5 mL
Product Image-PIER90067

Protein quantitation reagent, TMTzero™ label reagent

Catalogus nummer: (PIER90067)
Leverancier: Thermo Fisher Scientific
Omschrijving: Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.
UOM: 1 * 1 Kit
Product Image-786-154

Detergent removal system, DetergentOUT™ GBS10

Leverancier: G-Biosciences
Omschrijving: Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

Bel voor prijs
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
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