U heeft gezocht op: Chloroform-[D\\u2081]

Leverancier: VWR Chemicals

Omschrijving: Packaged at pH 6.6, this mixture is provided with a supplemental alkaline buffer that allows the researcher to easily increase the pH to 7.9 for DNA purification. When mixed with Chloroform:Isoamyl Alcohol (24:1), it makes an optimised reagent for DNA purification. Doubly distilled, high purity phenol packaged under nitrogen. Safer, easier alternative to crystalline phenol.

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Catalogus nummer: (ROCKMB-102-0100)

Leverancier: Rockland Immunochemicals

Omschrijving: DNA from calf thymus is commonly used in the preparation of pre-hybridisation and hybridisation solutions. Ideal for DNA polymerase assays. These prepared solutions are used to block the non-specific attachment of probe to the surface of a membrane. The DNA solutions are prepared from highly pure, phenol/chloroform-extracted DNA, and DNase- and RNase-free (DEPC-treated) distilled, deionised water.

UOM: 1 * 100 mg

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Leverancier: MP Biomedicals

Omschrijving: Soluble in methanol (50 mg/ml - clear, colourless solution), DMSO (50 mg/ml - clear, colourless solution), chloroform and ethanol. DMSO solutions can be stored aliquoted and frozen at −20 °C for several months. Paclitaxel undergoes transesterification in methanol, and it hydrolyses in aqueous solutions; Hardly soluble in water.

Catalogus nummer: (REST36280)

Leverancier: Restek

Omschrijving: The solvents consists of (C6) n-hexane (290 μg/ml), chloroform (60 μg/ml), 1,2-dimethoxyethane (100 μg/ml), 2-hexanone (50 μg/ml), nitromethane (50 μg/ml), pyridine (200 μg/ml), tetralin (100 μg/ml), trichloroethene (80 μg/ml).

UOM: 1 * 1 mL

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Leverancier: MP Biomedicals

Omschrijving: Soluble in acetone and in methanol (5 mg/ml) yielding a clear to very slightly hazy, yellow to amber solution. Gossypol is insoluble in water, very slightly soluble in petroleum ether and soluble in ethanol, ether, chloroform and DMF. It is freely soluble (with slow decomposition) in dilute aqueous solutions of ammonia and sodium carbonate.

Catalogus nummer: (786-133)

Leverancier: G-Biosciences

Omschrijving: Arrest™ Extraction Buffer is an optimised combination of various chaotropic agents and RNase inhibitors, which inhibits RNase rapidly. It may be used in conjunction with any RNA extraction method, including extractions based on phenol, chloroform and other organic solvents and detergents.

UOM: 1 * 1 EA

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Catalogus nummer: (EDVO202)

Leverancier: EDVOTEK

Omschrijving: Snelle isolatie van plasmide-DNA op kleine schaal is een routineprocedure die wordt gebruikt voor screening en analyse van recombinant DNA bij experimenten met klonen en subklonen. In dit experiment isoleren leerlingen plasmide-DNA zonder toxische chemicaliën zoals fenol of chloroform te gebruiken.

UOM: 1 * 1 Kit

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Catalogus nummer: (REST36270)

Leverancier: Restek

Omschrijving: The european pharmacopoeia/ICH Class 2 mix B, revised consists of 10 components, they are acetonitrile (410 μg/ml), chloroform (60 μg/ml), 1,2-dimethoxyethane (100 μg/ml), N,N-dimethylacetamide (1,090 μg/ml), 1,4-dioxane (380 μg/ml), 2-hexanone (50 μg/ml), methanol (3,000 μg/ml), nitromethane (50 μg/ml), pyridine (200 μg/ml) and tetralin (100 μg/ml).

UOM: 1 * 1 ST

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Leverancier: MP Biomedicals

Omschrijving: Trichloroacetic acid is used in protein precipitation; has been used to determine protein concentration by quantitative precipitation. Used as a decalcifier and fixative in microscopy. A protocol for the precipitation of nucleic acids can be found in Molecular Cloning.
Stability: Long term storage of aqueous solutions of less than 30% strength is not recommended. Decomposition products are chloroform, hydrochloric acid, carbon dioxide and carbon monoxide. Chloride ≤0.001% ; Nitrate ≤0.002%; Phosphate ≤5 ppm; Sulfate ≤0.02%

Catalogus nummer: (R6830-02)

Leverancier: OMEGA BIO-TEK

Omschrijving: RNA-Solv® Reagent is a one reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is a modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenised and lysed in RNA-Solv® Reagent, which maintains the integrity of the RNA while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

UOM: 1 * 200 mL

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Leverancier: Thermo Fisher Scientific

Omschrijving: GeneJET™ Whole Blood Genomic DNA Purification Mini Kit utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.

Leverancier: MP Biomedicals

Omschrijving: Below 35 to 40 °C gelatin swells and absorbs approximately 5 to 10 times its weight of water to form a gel. Gelatin is soluble in glycerol and acetic acid, and more soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulphide, carbon tetrachloride, ether, benzene, acetone and oils. Sterile solutions of gelatin stored cold are stable indefinitely, but at elevated temperatures hydrolysis or rupture of peptide bonds occurs, increasing the number of free amino groups. Gel strength and viscosity gradually weaken upon prolonged heating in solution above ~40 °C; this degradation is accelerated by extremes in pH, proteolytic enzymes and bacterial action.

Leverancier: OMEGA BIO-TEK

Omschrijving: The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from a small amount of cultured eukaryotic cells or tissues. This kit allows single or simultaneous processing of multiple samples in less than 40 minutes. Normally, 1×10⁷ eukaryotic cells or 25 to 30 mg tissue can be used in a single experiment. There is no need for phenol/chloroform extractions, time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and<i> in vitro</i> translation.

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Leverancier: OMEGA BIO-TEK

Omschrijving: The E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5×10⁶ cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilising HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.

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Catalogus nummer: (K0761)

Leverancier: Thermo Fisher Scientific

Omschrijving: GeneJET™ Whole Blood RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from whole blood and related body fluids. The kit utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 15 minutes. The purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analysis.

UOM: 1 * 1 Kit

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Catalogus nummer: (D2492-03)

Leverancier: OMEGA BIO-TEK

Omschrijving: The E.Z.N.A.® Blood DNA Maxi Kit is specially designed for large scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 10 ml whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.® Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.

UOM: 1 * 1 EA

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