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Product Image-733-2102

AccuStart™ Genotyping ToughMix®

Leverancier: Quantabio
Omschrijving: AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.

MSDS

Image Unavailable-733-2242

AccuStart™ II PCR SuperMix

Leverancier: Quantabio
Omschrijving: AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

MSDS

Product Image-TNB-6611-KIT

APO-BRDU™ kit, TUNEL Assay

Catalogus nummer: (TNB-6611-KIT)
Leverancier: Tonbo Biosciences
Omschrijving: The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.
UOM: 1 * 1 EA
Product Image-733-2265

PerfeCTa® PreAmp 5X SuperMix

Catalogus nummer: (733-2265)
Leverancier: Quantabio
Omschrijving: PerfeCTa® PreAmp SuperMix is een 5X geconcentreerde, gebruiksklare reactiecocktail voor zuivere, geselecteerde verrijking van doelsequenties uit beperkte hoeveelheden beginmateriaal voor downstream-genexpressieprofilering of gerichte resequencing. De cocktail bevat alle componenten, behalve primers en sjablonen. De 5X geconcentreerde SuperMix maakt toevoeging mogelijk van hogere sjabloonvolumes bij het werken met lage-concentratiemonsters en/of verminderde reactievolumes. Toevoeging van een inerte lichtblauwe traceerkleurstof helpt bij het visualiseren van kleine reactievolumes en zorgt voor nauwkeurig pipetteren.
UOM: 1 * 1 EA
Image Unavailable-GREI770280_960

Gel loading pipette tips

Catalogus nummer: (GREI770280_960)
Leverancier: Greiner Bio-One
Omschrijving: These gel loading tips are characterised by an elongated end. They are ideal for numerous molecular biological applications, especially for filling the slots of sequencing, PCR and protein electrophoresis gels.
UOM: 1 * 960 ST
Image Unavailable-733-2255

AccuStart™ II Taq DNA polymerase

Leverancier: Quantabio
Omschrijving: AccuStart™ II <i>Taq</i> DNA Polymerase is a high purity, recombinant <i>Taq</i> DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.

MSDS Certificaten

Product Image-BE-314

Experimentation kits, mutation detection and analysis

Catalogus nummer: (BE-314)
Leverancier: G-Biosciences
Omschrijving: A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental. Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations. The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping. Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.
UOM: 1 * 1 Kit
Product Image-F-547S

Direct PCR Kits

Leverancier: FINNZYMES REAGENTS
Omschrijving: Direct PCR Kits save time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Thermo Scientific Phire® Hot Start II DNA Polymerase and Phusion® Hot Start II High-Fidelity DNA Polymerase. These fusion enzymes are exceptionally fast, robust and highly tolerant of many PCR inhibitors present in unpurified sample material. This allows reliable amplification in challenging conditions where conventional DNA polymerases are completely inhibited.

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AccuStart™ II GelTrack PCR SuperMix

Leverancier: Quantabio
Omschrijving: AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II <i>Taq</i> DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

MSDS Certificaten

Product Image-TNB-6600-KIT

Anti-BrdU Mouse Monoclonal Antibody (FITC) [clone: PRB-1]

Catalogus nummer: (TNB-6600-KIT)
Leverancier: Tonbo Biosciences
Omschrijving: The fragmentation of genomic DNA by cellular nucleases during the later stages of apoptosis is also one of the most easily measured features of apoptotic cells. Nuclease activity generates DNA fragments ranging from ~300 bp to 50 bp in length, resulting in a typical DNA ‘laddering’ appearance when analyzed by agarose gel electrophoresis. These fragments have exposed 3’-hydroxyl (OH) ends which can be labeled with bromolated deoxyuridine triphosphates (Br-dUTP). An enzyme, terminal deoxynucleotidyl transferase (TdT), is used to catalyze the template-independent addition of Br-dUTP to the 3’-OH ends of double or single stranded DNA. This method is often called TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) or end labeling. Sites where the Br-dUTP is incorporated can then be detected with an antibody specific to BrdU. With the APO-BrdUTM Kit cells are first labeled with Br-dUTP, and then sites of incorporation are detected through staining with a FITC anti-BrdU antibody. Samples can then be analyzed via flow cytometry. Samples that are apoptotic will stain brightly with the anti-BrdU antibody due to the substantial number of exposed 3’-OH sites, while cells that are non-apoptotic will not have incorporated significant amounts of Br-dUTP and will stain dimly. The APO-BrdU Kit is shipped in one container and consists of two packages. Upon arrival one should be stored at 2-8°C and the other at -20°C.
UOM: 1 * 1 EA
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PCR mix, ready to use, Taq-&GO™, MP Biomedicals

Catalogus nummer: (ICNA11EPTAG100)
Leverancier: MP Biomedicals
Omschrijving: Taq-&GO™ is a ready to use optimised reaction mix for PCR.
UOM: 1 * 1 mL
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Master mixes, hot start, Phusion® U

Leverancier: FINNZYMES REAGENTS
Omschrijving: Phusion® U hot start PCR master mix is a novel engineered high fidelity enzyme developed using fusion technology.

Product Image-F-555L

DNA polymerases, Phusion® U hot start

Leverancier: FINNZYMES REAGENTS
Omschrijving: Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Product Image-27-0798-04

Recombinant Taq DNA Polymerase

Leverancier: Cytiva
Omschrijving: For PCR and other procedures requiring DNA polymerase activity at elevated temperatures.

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KOD Hot start master mix

Leverancier: Merck Millipore (Novagen)
Omschrijving: Premixed 2X KOD Hot Start PCR components for convenience and reproducibility.

Product Image-F-556S

DNA polymerases, Phusion® green U hot start

Leverancier: FINNZYMES REAGENTS
Omschrijving: Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Bel voor prijs
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
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