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Leverancier: Thermo Fisher Scientific

Omschrijving: RevertAid™ H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid™ H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Leverancier: Thermo Fisher Scientific

Omschrijving: The Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer and is supplemented with ROX passive reference dye. Only template and primers need to be added. Maxima™ hot start <i>Taq</i> DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Leverancier: Thermo Fisher Scientific

Omschrijving: FastDigest XbaI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest green reaction buffers.

Leverancier: Thermo Fisher Scientific

Omschrijving: The IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose

Leverancier: Thermo Fisher Scientific

Omschrijving: Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

Catalogus nummer: (FD0663)

Leverancier: Thermo Fisher Scientific

Omschrijving: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

UOM: 1 * 1 Kit

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Leverancier: Thermo Fisher Scientific

Omschrijving: Oligo(dT)₁₈ primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends. The primer is supplied as a ready to use, 20X concentrated aqueous solution. Oligo(dT)₁₈ primer has been functionally tested in first strand cDNA synthesis.

Leverancier: Thermo Fisher Scientific

Omschrijving: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Catalogus nummer: (EP0282)

Leverancier: Thermo Fisher Scientific

Omschrijving: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>.

UOM: 1 * 1 Kit

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Catalogus nummer: (FD1254)

Leverancier: Thermo Fisher Scientific

Omschrijving: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

UOM: 1 * 1 Kit

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Catalogus nummer: (EL0011)

Leverancier: Thermo Fisher Scientific

Omschrijving: DNA LIGASE T4 1000 UNITS (5U/µL) 1 * 1 Kit

UOM: 1 * 1 Kit

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Catalogus nummer: (K0503)

Leverancier: Thermo Fisher Scientific

Omschrijving: KIT GENEJET PLASMID MINIPREP 250RXN 1 * 1 Kit

UOM: 1 * 1 Kit

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Catalogus nummer: (EO0491)

Leverancier: Thermo Fisher Scientific

Omschrijving: PROTEINASE K RECOMB. PCR GRADE 1ML 1 * 1 Kit

UOM: 1 * 1 Kit

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Catalogus nummer: (EN0531)

Leverancier: Thermo Fisher Scientific

Omschrijving: RNASE A DNASE AND PROTEASE FREE 10MG 1 * 1 Kit

UOM: 1 * 1 Kit

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Catalogus nummer: (EF0651)

Leverancier: Thermo Fisher Scientific

Omschrijving: FASTAP THERMOSENS ALK PHOS 1000 UNITS 1 * 1 Kit

UOM: 1 * 1 Kit

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Catalogus nummer: (EN0521)

Leverancier: Thermo Fisher Scientific

Omschrijving: DNASE I RNASE-FREE 1000U (1U/µL) 1 * 1 Kit

UOM: 1 * 1 Kit

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