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Leverancier: G-Biosciences
Omschrijving: Streptavidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵ M⁻¹) and streptavidin (Ka=10¹⁵ M⁻¹). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3M guanidinehydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH 1,5 or by autoclaving. The biotinylated molecules are efficiently probed with avidin or streptavidin conjugated to reporter molecules, such as peroxidases or phosphatases.

Leverancier: G-Biosciences
Omschrijving: Guanidinium thiocyanate

Catalogus nummer: (786-453)
Leverancier: G-Biosciences
Omschrijving: The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.
UOM: 1 * 1 Kit


Catalogus nummer: (786-488)
Leverancier: G-Biosciences
Omschrijving: Potassium dihydrogen phosphate 1 mol/l aqueous solution
UOM: 1 * 1 L


Catalogus nummer: (786-680)
Leverancier: G-Biosciences
Omschrijving: Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.
UOM: 1 * 50 mL


Catalogus nummer: (786-678)
Leverancier: G-Biosciences
Omschrijving: Swift™ Film Cleaner allows researchers to clean film that has been overexposed or has a high background or speckling without having to repeat experiments. Suitable for all exposed film developed for gel shift assays, Western, Southern and Northern blots.
UOM: 1 * 1 Kit


Catalogus nummer: (786-514)
Leverancier: G-Biosciences
Omschrijving: Triton® X-100 is bedoeld voor het oplosbaar maken van membraaneiwitten tijdens de isolatie van membraan-eiwitcomplexen.
UOM: 1 * 1 L


Catalogus nummer: (BE-502)
Leverancier: G-Biosciences
Omschrijving: Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.
UOM: 1 * 1 Kit


Catalogus nummer: (BE-314)
Leverancier: G-Biosciences
Omschrijving: A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental. Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations. The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping. Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.
UOM: 1 * 1 Kit


Catalogus nummer: (BE-318)
Leverancier: G-Biosciences
Omschrijving: The kit utilises the principle of diffusion of nucleic acids on a nylon membrane to determine their concentration. No spectrophotometers required.
UOM: 1 * 1 Kit


Catalogus nummer: (786-022)
Leverancier: G-Biosciences
Omschrijving: FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.
UOM: 1 * 1 Kit


Catalogus nummer: (ML-04)
Leverancier: G-Biosciences
Omschrijving: GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.
UOM: 1 * 1 EA


Catalogus nummer: (786-126)
Leverancier: G-Biosciences
Omschrijving: The Column-PROTEIN-Concentrate™ Kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilises any protein in a low salt buffer between pH 2 to 12. The binding capacity is ~0,5 mg protein/ml Protein Binding Resin.
UOM: 1 * 1 Kit

MSDS


Catalogus nummer: (API-06)
Leverancier: G-Biosciences
Omschrijving: Apoptosis is commonly defined as programmed mechanism of cell death and is a highly regulated process that can be initiated by a variety of internal and external stimuli (inducers).
UOM: 1 * 50 µl


Catalogus nummer: (786-398)
Leverancier: G-Biosciences
Omschrijving: OmniPrep™ for Gram Positive Bacteria Kit isolates high quality genomic DNA from gram-positive bacteria. If used according to the protocols, this kit purifies DNA from 100 ml gram-positive bacteria culture.
UOM: 1 * 1 Kit


Catalogus nummer: (786-651)
Leverancier: G-Biosciences
Omschrijving: β-Galactosidase assay is for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. It uses chlorophenol red-β-D-galactopyranoside (CPRG), which is up to 10 times more sensitive than the classic O-nitrophenyl-β-D-galactopyranoside (ONPG) β-Galactosidase assays. This increased sensitivity makes it simpler to measure β-galactosidase activity in cells that are difficult to transfect or have low β-galactosidase activity expression. β-Galactosidase activity is measured using a spectrophotometer or a microplate reader to determine the amount of substrate converted at 570 to 595 nm.
UOM: 1 * 1 EA


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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