U heeft gezocht op: Merck Millipore (Novagen)

Leverancier: Merck Millipore (Novagen)

Omschrijving: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Catalogus nummer: (69909-3)

Leverancier: Merck Millipore (Novagen)

Omschrijving: The pET-30a-c(+) vectors carry an N-terminal His Tag®/thrombin/S Tag™/enterokinase configuration plus an optional C-terminal His tag sequence.

UOM: 1 * 1 EA

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Leverancier: Merck Millipore (Novagen)

Omschrijving: PopCulture® Reagent is a detergent-based concentrate that can be added directly to cultures of <i>E. coli</i> to effectively extract proteins without the need for cell harvest. Recombinant proteins can be directly screened in the crude extract, or purified by adding an affinity matrix, washing the matrix-target protein complex to remove spent culture medium and cellular contaminants, and eluting the purified protein from the matrix. The PopCulture protein purification procedure is ideally suited to high-throughput robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.

Leverancier: Merck Millipore (Novagen)

Omschrijving: pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.

Catalogus nummer: (70793-3)

Leverancier: Merck Millipore (Novagen)

Omschrijving: BugBuster®Ni-NTA HIS-BIND® Purification Kit is used for protein purification. Ni-NTA HIS-BIND® Resin is high-performance Ni2+-charged agarose used for rapid, one-step purification of proteins containing a polyhistidine tag sequence.

UOM: 1 * 1 Kit

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Leverancier: Merck Millipore (Novagen)

Omschrijving: Ni-NTA HisBind® Superflow™ Resin is a medium-pressure compatible version of the Ni-NTA HisBind® Resin

Leverancier: Merck Millipore (Novagen)

Omschrijving: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

Catalogus nummer: (71610-3)

Leverancier: Merck Millipore (Novagen)

Omschrijving: D-Desthiobiotin provides a gentle elution of StrepTag® II proteins from the biotin-binding site of StrepTactin® resins. D-Desthiobiotin is offered as a lyophilised powder for preparation of elution buffer.

UOM: 1 * 1 g

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Leverancier: Merck Millipore (Novagen)

Omschrijving: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Catalogus nummer: (71366-4)

Leverancier: Merck Millipore (Novagen)

Omschrijving: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

UOM: 1 * 1 ST

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Leverancier: Merck Millipore (Novagen)

Omschrijving: BugBuster® 10X is een geconcentreerde formule van de gepatenteerde reinigingsmiddelen die in BugBuster® worden gebruikt zonder de toevoeging van zouten of buffercomponenten. Geconcentreerd BugBuster® biedt een flexibel alternatief voor de gebruiksklare 1X BugBuster®, waarbij de gebruiker het reagens zelf kan verdunnen of buffercomponenten kan toevoegen. BugBuster® 10X biedt alle voordelen van standaard-BugBuster® plus de vrijheid om de vereiste pH, reagensconcentratie en bufferadditieven te bepalen voor de maximale extractie en activiteit van uw doeleiwit.

Leverancier: Merck Millipore (Novagen)

Omschrijving: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Leverancier: Merck Millipore (Novagen)

Omschrijving: Novagen® T7Select® Phage Display System takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0,1 to 1 per phage) or mid-copy number (5 to 15 per phage).

Leverancier: Merck Millipore (Novagen)

Omschrijving: Insect GeneJuice®-transfectiereagens is speciaal geformuleerd om de transfectie van Sf9 insectencellen uit te voeren met maximale efficiëntie. Dit reagens vertoont een zeer lage toxiciteit ten opzichte van de cellen en kan worden gebruikt voor zowel korte als stabiele transfecties in serumbevattende of serumvrije media. Verder kan het reagens worden gebruikt voor de cotransfectie van transferplasmides met gelineariseerd virus DNA voor de productie van recombinante baculovirussen.

Leverancier: Merck Millipore (Novagen)

Omschrijving: THP [Tris(hydroxypropyl)phosphine; C9H21O3P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT).

Leverancier: Merck Millipore (Novagen)

Omschrijving: Lysonase™ bioprocessing reagent is an optimised, ready-to-use convenient blend of rLysozyme™ solution and benzonase nuclease which is ideal for protein extraction.

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