U heeft gezocht op: End-point PCR Enzymes and Kits

Catalogus nummer: (71975-3)

Leverancier: Merck Millipore (Novagen)

Omschrijving: KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA templates.

UOM: 1 * 200 PE

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Leverancier: MP Biomedicals

Omschrijving: The Taq CORE kits are designed for the amplification of specific regions of DNA using PCR. The PCR CORE Kits - Direct Loading provide <i>Taq</i> DNA Polymerase, PCR Direct Loading buffers and dNTPs necessary for amplification and subsequent loading of samples on an agarose gel.

Leverancier: Merck Millipore (Novagen)

Omschrijving: KOD HiFi DNA-polymerase is een uniek proofreading-enzym dat is geïsoleerd uit de extreem thermofiele <i>Thermococcus kodakaraensis </i>KOD1. Het heeft een superieure processiviteit en betrouwbaarheid, waardoor snellere en nauwkeurigere PCR-amplificatie mogelijk is dan met conventionele enzymen.

Leverancier: G-Biosciences

Omschrijving: <i>Pfu</i> DNA Polymerase, derived from the hyperthermophilic archae <i>Pyrococcus furiosus</i>, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). <i>Pfu</i> DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that<i> Pfu</i> DNA Polymerase-generated PCR fragments will have fewer errors than <i>Taq</i>-generated PCR inserts. Using <i>Pfu</i> DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. <i>Pfu</i> DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Leverancier: G-Biosciences

Omschrijving: <i>Taq</i> DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile <i>Thermus aquaticus</i>. The molecular weight of the recombinant protein is 94 kD. The <i>Taq</i> Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this <i>Taq</i> Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. <i>Taq</i> DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Catalogus nummer: (ICNA11EPTAG100)

Leverancier: MP Biomedicals

Omschrijving: Taq-&GO™ is a ready to use optimised reaction mix for PCR.

UOM: 1 * 1 mL

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Leverancier: Cytiva

Omschrijving: For PCR and other procedures requiring DNA polymerase activity at elevated temperatures.

Catalogus nummer: (25-1500-01)

Leverancier: Cytiva

Omschrijving: illustra™ Hot Start Master Mix is a 2X premixed formulation that can effectively reduce non specific priming and primer-dimer formation during PCR. illustra™ Hot Start Master Mix uses a novel hot start method called primer sequestration.

UOM: 1 * 1 Kit

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Leverancier: Cytiva

Omschrijving: PuReTaq RTG PCR beads and reagents ensures reliable and robust performance in both end point and RT-PCR amplifications and ensures the lowest possible levels of contaminating prokaryotic and eukaryotic nucleic acids.

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Leverancier: Cytiva

Omschrijving: illustra™ Hot Start Mix, Ready-To-Go™ is a pre-formulated and pre-dispensed, freeze-dried PCR reagent mix, including high quality PuReTaq™ DNA polymerase and a hot start activator protein, for increased specificity and reproducibility of PCR amplifications. The Ready-To-Go™ beads are provided in either 0,5 or 0,2 ml tubes that are compatible with most thermal cyclers.