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Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.


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Product Image-M334-100ML

VWR® Lysis Buffer, Hypotonic

Catalogus nummer: (M334-100ML)
Leverancier: VWR Chemicals
Omschrijving: Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.
UOM: 1 * 100 mL
Product Image-G193-09

J.T.Baker® Cell Lysis Solution, Biotech Reagent

Leverancier: Avantor
Omschrijving: J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

Environmentally Preferable

Product Image-TNB-4300-L100

RBC Lysis Buffer (10X)

Catalogus nummer: (TNB-4300-L100)
Leverancier: Tonbo Biosciences
Omschrijving: RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.
UOM: 1 * 100 mL

Certificaten


Image Unavailable-ENZOADI801496

Cell lysis buffer, RIPA

Catalogus nummer: (ENZOADI801496)
Leverancier: ENZO LIFE SCIENCES
Omschrijving: The RIPA liquid cell lysis buffer composes of 50 mM TRIS-hydrogen chloride, pH 7,4, containing 150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 1,2% Triton X-100, 0,5% sodium deoxycholate, and 0,1% SDS.
UOM: 1 * 100 mL

New Product


Product Image-786-580

Gram Negative lysis buffer

Leverancier: G-Biosciences
Omschrijving: Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including <i>E.coli</i> strains.

Image Unavailable-R0424-02

E.Z.N.A.® RNALock stabilizer reagent

Leverancier: OMEGA BIO-TEK
Omschrijving: The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.

MSDS Certificaten

Image Unavailable-ENZOADI801339

Cell lysis buffer 4, (100 ml), Enzo Life Sciences

Catalogus nummer: (ENZOADI801339)
Leverancier: ENZO LIFE SCIENCES
Omschrijving: Cell lysis buffer 4, Enzo Life Sciences
UOM: 1 * 100 mL

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Image Unavailable-AGLS400900

Cell lysis analysis kit, SideStep II

Catalogus nummer: (AGLS400900)
Leverancier: AGILENT
Omschrijving: Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.
UOM: 1 * 1 ST

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Product Image-X193-01

J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent

Leverancier: Avantor
Omschrijving: J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.

Certificaten Environmentally Preferable

Image Unavailable-TCIAB2904-100ML

Guanidine hydrochloride buffer solution, 6 mol/L, pH 8,7

Catalogus nummer: (TCIAB2904-100ML)
Leverancier: TCI
Omschrijving: Guanidine Hydrochloride Buffer Solution, 6 mol/L, pH-waarde: 8,7, TCI
UOM: 1 * 100 mL
Image Unavailable-ENZOADI801526

Extraction reagents

Leverancier: ENZO LIFE SCIENCES
Omschrijving: Concentrated lysis buffer for preparation of cell and tissue extracts.

New Product

Image Unavailable-ENZOBMLKI1170030

Caspase cell lysis buffer

Catalogus nummer: (ENZOBMLKI1170030)
Leverancier: ENZO LIFE SCIENCES
Omschrijving: Caspase cell lysis buffer, Enzo Life Sciences
UOM: 1 * 30 mL

New Product


Product Image-786-033

Research buffers and reagents

Leverancier: G-Biosciences
Omschrijving: These DNase- and RNase-free buffers and reagents are designed for high quality research.

Image Unavailable-ENZOADI801284

RIPA cell lysis buffer 2

Catalogus nummer: (ENZOADI801284)
Leverancier: ENZO LIFE SCIENCES
Omschrijving: RIPA cell lysis buffer 2, Enzo Life Sciences
UOM: 1 * 100 mL

New Product


Product Image-786-181

Protein extraction kit, tissue PE LB™

Leverancier: G-Biosciences
Omschrijving: Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.

Product Image-78840.

Subcellular Protein Fractionation Kit for Cultured Cells

Catalogus nummer: (78840.)
Leverancier: Thermo Fisher Scientific
Omschrijving: This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.
UOM: 1 * 1 Kit
Bel voor prijs
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
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