U heeft gezocht op: Reagentia voor eiwitextractie

Catalogus nummer: (ENZOBMLKI1050300)

Leverancier: ENZO LIFE SCIENCES

Omschrijving: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).

UOM: 1 * 300 µl

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Leverancier: G-Biosciences

Omschrijving: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

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Leverancier: G-Biosciences

Omschrijving: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Leverancier: G-Biosciences

Omschrijving: OrgoSol DetergentOUT™ is suitable for removal of detergents from protein solutions, including hydrophobic protein solutions. Compatible with all detergent types, its performance is not dependent on the concentration of detergents in the solution. It is highly flexible and can be used to process small and large sample volumes.

Catalogus nummer: (PIER90051)

Leverancier: Thermo Fisher Scientific

Omschrijving: Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.

UOM: 1 * 1 EA

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Catalogus nummer: (786-428)

Leverancier: G-Biosciences

Omschrijving: Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.

UOM: 1 * 0,25 mL

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Catalogus nummer: (PIER84841)

Leverancier: Thermo Fisher Scientific

Omschrijving: The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.

UOM: 1 * 1 EA

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Catalogus nummer: (PIER20343)

Leverancier: Thermo Fisher Scientific

Omschrijving: Immobilised Pepsin agarose consists of an acidic endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.

UOM: 1 * 5 mL

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Leverancier: Thermo Fisher Scientific

Omschrijving: Pierce™ Detergent removal resin enables removal of commonly used ionic, non-ionic and zwitterionic detergents from protein/peptide digest solutions.

Leverancier: Thermo Fisher Scientific

Omschrijving: The HiPPR™ Detergent Removal Spin Column Kit improves mass spectrometry results by efficiently removing detergents from 25 to 200 µl samples with low protein or peptide concentrations.

Catalogus nummer: (PIER20341)

Leverancier: Thermo Fisher Scientific

Omschrijving: Pierce™ Immobilised Papain consists of a cysteine-endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.

UOM: 1 * 5 mL

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Leverancier: MP Biomedicals

Omschrijving: Lysing Matrix Z is used primarily for lysis of animal and plant tissue, tougher stems, roots and seeds, and whole insects. Each impact-resistant tube contains yttria-stabilised zirconium oxide beads.

Catalogus nummer: (PIER88216)

Leverancier: Thermo Fisher Scientific

Omschrijving: This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

UOM: 1 * 1 ST

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Catalogus nummer: (786-791)

Leverancier: G-Biosciences

Omschrijving: A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.

UOM: 1 * 1 ST

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Catalogus nummer: (786-790)

Leverancier: G-Biosciences

Omschrijving: A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.

UOM: 1 * 1 ST

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Catalogus nummer: (PIER20308)

Leverancier: Thermo Fisher Scientific

Omschrijving: SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0,1 to 10 ml) protein samples.

UOM: 1 * 1 Kit

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