U heeft gezocht op: Reagentia voor eiwitextractie

Leverancier: Thermo Fisher Scientific

Omschrijving: Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.

MSDS

Leverancier: Thermo Fisher Scientific

Omschrijving: The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) Label Reagents enable multiplexed characterisation and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidised proteins) by mass spectrometry (MS).

Catalogus nummer: (PIER28340)

Leverancier: Thermo Fisher Scientific

Omschrijving: Surfact-Amps™ Detergent sampler is a collection of various Surfact-Amps™ detergent solutions and selected other useful detergents for protein research methods.

UOM: 1 * 1 Kit

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Catalogus nummer: (PIER20230)

Leverancier: Thermo Fisher Scientific

Omschrijving: Pierce™ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.

UOM: 1 * 2 mL

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Catalogus nummer: (786-792)

Leverancier: G-Biosciences

Omschrijving: Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.

UOM: 1 * 1 ST

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Catalogus nummer: (324713-50)

Leverancier: Merck Millipore (Calbiochem‎)

Omschrijving: Native endoproteinase Glu-C from <i>Staphylococcus aureus</i>. Serine protease that specifically hydrolyses peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

UOM: 1 * 1 EA

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Leverancier: Merck Millipore (Novagen)

Omschrijving: PopCulture® Reagent is a detergent-based concentrate that can be added directly to cultures of <i>E. coli</i> to effectively extract proteins without the need for cell harvest. Recombinant proteins can be directly screened in the crude extract, or purified by adding an affinity matrix, washing the matrix-target protein complex to remove spent culture medium and cellular contaminants, and eluting the purified protein from the matrix. The PopCulture protein purification procedure is ideally suited to high-throughput robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.

Leverancier: Merck Millipore (Novagen)

Omschrijving: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Leverancier: Merck Millipore (Novagen)

Omschrijving: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Leverancier: Thermo Fisher Scientific

Omschrijving: Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

Certificaten

Catalogus nummer: (324708-2)

Leverancier: Merck Millipore (Calbiochem‎)

Omschrijving: Native endoproteinase Asp-N from <i>Pseudomonas fragi.</i> Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

UOM: 1 * 1 EA

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Catalogus nummer: (786-680)

Leverancier: G-Biosciences

Omschrijving: Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.

UOM: 1 * 50 mL

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Catalogus nummer: (REST25849)

Leverancier: Restek

Omschrijving: QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.

UOM: 1 * 50 ST

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Leverancier: VARIAN

Omschrijving: Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.

Catalogus nummer: (CEMATMS001-4X4L)

Leverancier: CEM

Omschrijving: Modern peptide synthesis agents can dramatically reduce waste generation, but challenges remain regarding the health and environmental impacts of commonly used solvents.

UOM: 1 * 4 L

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Catalogus nummer: (WATE186001861)

Leverancier: WATERS

Omschrijving: RapiGest™ SF is an anionic surfactant for peptide digestion. Supplied as five 1 mg vials for 1 to 50 digestions. The digestion speed and more peptide recovery is enhanced by using trypsin or other enzymes.

UOM: 1 * 5 ST

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