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Catalogus nummer: (PIER28340)

Leverancier: Thermo Fisher Scientific

Omschrijving: Surfact-Amps™ Detergent sampler is a collection of various Surfact-Amps™ detergent solutions and selected other useful detergents for protein research methods.

UOM: 1 * 1 Kit

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Leverancier: Thermo Fisher Scientific

Omschrijving: Detoxi-Gel™ endotoxin removing columns and gel uses polymixin B ligand immobilised on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.

Catalogus nummer: (PIER20230)

Leverancier: Thermo Fisher Scientific

Omschrijving: Pierce™ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.

UOM: 1 * 2 mL

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Catalogus nummer: (786-232)

Leverancier: G-Biosciences

Omschrijving: The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.

UOM: 1 * 1 Kit

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Catalogus nummer: (786-792)

Leverancier: G-Biosciences

Omschrijving: Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.

UOM: 1 * 1 ST

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Catalogus nummer: (PIER90003)

Leverancier: Thermo Fisher Scientific

Omschrijving: Pierce™ Phosphoprotein Enrichment Kit is designed for the efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues.

UOM: 1 * 10 ST

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Leverancier: Thermo Fisher Scientific

Omschrijving: Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

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Catalogus nummer: (PIER89839)

Leverancier: Thermo Fisher Scientific

Omschrijving: Organelle enrichment kit for lysosomes enables enrichment of intact organelles from cells and tissue. The kit provides the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation.

UOM: 1 * 1 Kit

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Catalogus nummer: (PIER89874)

Leverancier: Thermo Fisher Scientific

Omschrijving: The Mitochondria isolation Kit for cultured cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes.

UOM: 1 * 1 Kit

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Leverancier: Merck Millipore (Novagen)

Omschrijving: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Leverancier: Merck Millipore (Novagen)

Omschrijving: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Leverancier: Merck Millipore (Calbiochem‎)

Omschrijving: ProteoExtract® Kits cover the different steps of the proteomics workflow, from protein extraction and abundant protein removal to concentration of protein mixtures and removal of interfering substances, digestion of proteins and selective capturing of phosphopeptides. These kits work in concert with one another to provide protein samples that are directly compatible with downstream analytical applications.

Catalogus nummer: (324708-2)

Leverancier: Merck Millipore (Calbiochem‎)

Omschrijving: Native endoproteinase Asp-N from <i>Pseudomonas fragi.</i> Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

UOM: 1 * 1 EA

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Catalogus nummer: (786-126)

Leverancier: G-Biosciences

Omschrijving: The Column-PROTEIN-Concentrate™ Kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilises any protein in a low salt buffer between pH 2 to 12. The binding capacity is ~0,5 mg protein/ml Protein Binding Resin.

UOM: 1 * 1 Kit

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Leverancier: Merck Millipore (Novagen)

Omschrijving: BugBuster® Plus Lysonase™ bevat BugBuster®-reagens voor eiwitextractie en Lysonase-bioverwerkingsreagens, een geoptimaliseerd, gebruiksklaar mengsel van rLysozyme™ en Benzonase®-nuclease in één kit. Deze krachtige combinatie van twee reagentia maakt maximale terugwinning van actief oplosbaar eiwit uit Gram-negatieve en Gram-positieve bacteriën mogelijk. Met BugBuster en rLysozyme worden de bacteriële cellen op efficiënte wijze gelyseerd terwijl met Benzonase nucleïnezuren worden gedegradeerd voor verdere verwerking. Vereist 5 ml BugBuster en 10 µl Lysonase per 1 g celpasta. De kits bieden voldoende reagentia voor eiwitextractie uit 20 g of 100 g celpasta.

Catalogus nummer: (324715-5)

Leverancier: Merck Millipore (Calbiochem‎)

Omschrijving: Native endoproteinase Lys-C from <i>Lysobacter enzymogenes</i>. Serine protease that specifically hydrolyses amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease: protein by weight) for sequence analysis.

UOM: 1 * 1 EA

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