Caspase-3/7 Activity Plate Reader Assay Kit, Green

Leverancier: STEMCELL Technologies
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STMC100-0920EA 524 EUR
STMC100-0920
Caspase-3/7 Activity Plate Reader Assay Kit, Green
testen Cellulaire assays
For detection of caspase-3/7 activity in cell lysates prepared from mammalian cells undergoing apoptosis - 200 tests.

  • Z-DEVD-Rh 110-DVED-Z detects caspase-3/7 activity
  • Excitation wavelength: 500 nm | Emission wavelength: 522 nm
  • Extinction coefficient: 80,000 cm⁻¹M⁻¹
  • Easily scalable for high-throughput assays
  • Verified for use with fluorescent plate readers

Efficiently detect caspase-3/7 activity in cell lysates prepared from mammalian cells undergoing apoptosis by using the Caspase-3/7 Activity Plate Reader Assay Kit, Green, which includes the fluorogenic substrate Z-DEVD-Rh 110-DVED-Z and an assay buffer. This reagent kit uses a quick and simple staining protocol that is well suited for high-throughput screening and compatible with standard fluorescent plate readers.

Caspase-3 and caspase-7 have a proteolytic substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). When added to apoptotic cells, Z-DEVD-Rh 110-DVED-Z is cleaved by activated caspase-3/7, generating a green fluorescent signal that can be detected by standard plate readers.

Caspases are a family of proteases that play a central role in cellular apoptosis through the cleavage of select proteins, and result in the disassembly of the cell (Thornberry and Lazebnik Science, 1998). This process is switched on by pro-apoptotic signals from death receptors or stimuli such as cytotoxic reagents, which activate the initiator caspases and lead to activation of the effector caspases-caspase-3 and caspase-7 (Thornberry and Lazebnik Science, 1998). Caspase assays are useful for quantifying caspase-3/7 activity in apoptotic cells and for screening caspase-3/7 inhibitors.

CAS. number: 67-68-5
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