U heeft gezocht op: Leeractiviteiten voor biochemie

Catalogus nummer: (BE-404)

Leverancier: G-Biosciences

Omschrijving: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

UOM: 1 * 1 Kit

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Catalogus nummer: (BE-401)

Leverancier: G-Biosciences

Omschrijving: The Physical Properties of Proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

UOM: 1 * 1 Kit

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Catalogus nummer: (EDVO300)

Leverancier: EDVOTEK

Omschrijving: When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent <i>E. coli</i> cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.

UOM: 1 * 1 Kit

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Catalogus nummer: (EDVO266)

Leverancier: EDVOTEK

Omschrijving: Milk proteins are the most common food allergens in children. Accurate detection and labeling is vital to inform consumers about potentially dangerous foods.

UOM: 1 * 1 Kit

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Catalogus nummer: (EDVOS-47)

Leverancier: EDVOTEK

Omschrijving: Investigate how agarose gel electrophoresis unlocks the color code used by food scientists to make colorful candies.

UOM: 1 * 1 Kit

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Leverancier: EDVOTEK

Omschrijving: De drie meestgebruikte restrictie-enzymen zijn Eco RI, Bam HI en Hind III. Elk van deze enzymen versnelt het splitsen van DNA bij de gedefinieerde basesequentie. Alle enzymen zijn gelyofiliseerd en bevatten 1500 units. Eén unit wordt gedefinieerd als de hoeveelheid van een enzym die nodig is voor het verteren van 1,0 µg lambda-DNA in 60 minuten bij 37 °C in een reactiemengsel van in totaal 50 µl.

Catalogus nummer: (EDVO225)

Leverancier: EDVOTEK

Omschrijving: This experiment helps teach students about restriction enzyme digests in the context of forensic science. Students cut DNA with restriction enzymes and then compare the banding pattern of the crime scene DNA versus that of two suspects using agarose gel electrophoresis.

UOM: 1 * 1 Kit

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Catalogus nummer: (EDVO212)

Leverancier: EDVOTEK

Omschrijving: The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for <i>Eco</i> RI (5 distinct sites, 2 are very close in size). In this experiment, Lambda DNA is digested by the <i>Eco</i> RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.

UOM: 1 * 1 Kit

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Catalogus nummer: (EDVO202)

Leverancier: EDVOTEK

Omschrijving: Snelle isolatie van plasmide-DNA op kleine schaal is een routineprocedure die wordt gebruikt voor screening en analyse van recombinant DNA bij experimenten met klonen en subklonen. In dit experiment isoleren leerlingen plasmide-DNA zonder toxische chemicaliën zoals fenol of chloroform te gebruiken.

UOM: 1 * 1 Kit

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Catalogus nummer: (EDVO282)

Leverancier: EDVOTEK

Omschrijving: Via dit eenvoudige en veilige experiment kunnen leerlingen meer te weten komen over de katalyse van enzymen, de aard van de werking van enzymen en het verband tussen structuur en functie van proteïnen. De leerlingen voeren een enzymassay uit en bepalen de snelheid van de enzymatische reactie.

UOM: 1 * 1 Kit

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Catalogus nummer: (EDVO303)

Leverancier: EDVOTEK

Omschrijving: Vier experimentele modules zijn samengevoegd in één experiment voor een uitgebreid biotechnologieonderzoek gericht op de groene fluorescente proteïne (GFP). Bacteriële cellen worden getransformeerd om GFP af te scheiden. De getransformeerde cellen worden vervolgens op kweek gezet en de GFP wordt gezuiverd door middel van kolomchromatografie. Ten slotte wordt de zuiverheid van de proteïnefracties geanalyseerd door middel van elektroforese met SDS-polyacrylamide.

UOM: 1 * 1 Kit

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Catalogus nummer: (EDVO235)

Leverancier: EDVOTEK

Omschrijving: Microarray technology allows scientists to screen large numbers of samples in one assay. This technology allows high-throughput screening of small samples and allows comparisons to be made between healthy and unhealthy tissues. In this experiment, students apply simulated DNA and RNA samples to a membrane to screen for positive and negative samples.

UOM: 1 * 1 Kit

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Catalogus nummer: (EDVO203)

Leverancier: EDVOTEK

Omschrijving: Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from <i>E. coli</i>. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.

UOM: 1 * 1 Kit

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Catalogus nummer: (EDVO302)

Leverancier: EDVOTEK

Omschrijving: In dit experiment voeren de leerlingen een zuivering van het restrictie-enzym <i>Eco </i>RI uit. Bij deze procedure wordt gebruikgemaakt van een stap met ionenuitwisselingschromatografie voor het zuiveren van <i>Eco </i>RI. De kolomfracties worden getoetst op het enzym met behulp van lambda-DNA en de verteringsproducten worden geïdentificeerd door middel van gelelektroforese. Fracties die <i>Eco </i>RI bevatten, worden geïdentificeerd en gepoold. De totale en specifieke activiteit worden berekend. Aanbevolen voor cursussen voor gevorderden.

UOM: 1 * 1 Kit

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Catalogus nummer: (BE-406)

Leverancier: G-Biosciences

Omschrijving: Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.

UOM: 1 * 1 Kit

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Catalogus nummer: (BE-409)

Leverancier: G-Biosciences

Omschrijving: In this lab activity, students learn to perform carefully controlled experiments to generate protein fragments using a proteolysis enzyme and then analyse the protein fragments by electrophoresis. By analysis of protein fragmentation patterns, i.e. protein fingerprints, students learn about protein sequence, structure, and their conservation. Students resolve a set of 3 functionally identical protein samples selected from throughout the animal kingdom; including human, bovine and sheep. After generating fingerprints by electrophoresis, students examine the protein fingerprint of each sample to determine the degree of conservation.

UOM: 1 * 1 Kit

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