U heeft gezocht op: Reagentia voor nucleïnezuren

Catalogus nummer: (EP0072)

Leverancier: Thermo Fisher Scientific

Omschrijving: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>. Native<i> Taq</i> DNA polymerase stabilised with BSA is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.

UOM: 1 * 1 Kit

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Catalogus nummer: (AATB17043)

Leverancier: AAT BIOQUEST

Omschrijving: This iFluor® 647-PEG12-dUTP conjugate has the same spectral properties as ChromaTide™ Alexa Fluor™ 647-dUTP.

UOM: 1 * 1 EA

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Leverancier: Thermo Fisher Scientific

Omschrijving: RevertAid™ Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

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Catalogus nummer: (ICNA112200200)

Leverancier: MP Biomedicals

Omschrijving: Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transformation and has been successfully used to transform linear DNA into <i>Pichia pastoris</i> for protein expression studies.

UOM: 1 * 1 EA

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Catalogus nummer: (07EMC043)

Leverancier: MP Biomedicals

Omschrijving: MPure-32™ aNAP System is a fully automated magnetic bead-based nucleic acid extraction system. It can process up to 32 samples simultaneously within a short period of time (around 40 to 60 minutes). Besides its capability to extract nucleic acid with high purity and yield, MPure-32™ aNAP System is easy to use; this eliminates the risk of human error and cross-contamination.

UOM: 1 * 1 ST

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Catalogus nummer: (AATB67036)

Leverancier: AAT BIOQUEST

Omschrijving: TAMRA dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems

UOM: 1 * 100 µl

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Leverancier: AAT BIOQUEST

Omschrijving: TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

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Leverancier: FINNZYMES REAGENTS

Omschrijving: Phusion® U green multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Leverancier: FINNZYMES REAGENTS

Omschrijving: Phusion® green hot start II high-fidelity PCR master mix is a convenient 2X mix designed to minimise the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimised reaction buffer including MgCl₂. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.

Catalogus nummer: (AATB17258)

Leverancier: AAT BIOQUEST

Omschrijving: 10 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water.

UOM: 1 * 1 mL

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Catalogus nummer: (AATB17035)

Leverancier: AAT BIOQUEST

Omschrijving: Fluorescein-12-dCTP might be incorporated into a DNA sequence as its natural counterpart dCTP to incorporate a fluorescein tag in a DNA molecule.

UOM: 1 * 1 EA

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Catalogus nummer: (786-395)

Leverancier: G-Biosciences

Omschrijving: OmniPrep™ for Tissue Kit is to specifically isolate high quality genomic DNA from tissue samples, including fresh, frozen, fixed or paraffin-embedded tissue. The kit isolates DNA from bodily fluids, including plasma, serum, amniotic fluid, semen and CSF. Also isolates high quality DNA from cultured cells, non-mammalian nucleated blood and gram-negative bacteria.

UOM: 1 * 1 Kit

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Leverancier: G-Biosciences

Omschrijving: Tri-Xtract™ is designed for the isolation of total RNA that is free from protein and DNA contamination. The isolated RNA is suitable for Northern blots, dot blot hybridisation, <i>in vitro</i> translation, RNase protection assays and poly (A+) selection.

Leverancier: Thermo Fisher Scientific

Omschrijving: GeneJET™ Plasmid Midiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant <i>E. coli</i> cultures. The kit utilises silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing,<i> in vitro</i> transcription, and transfection of robust cell lines.

Leverancier: Biotium

Omschrijving: The DNA Gel Extraction Kit is a silica-gel, spin column based DNA extraction kit designed to purify 40 bp to 40 kb DNA fragments from agarose gels in TAE or TBE buffer.

MSDS

Leverancier: SGI - DNA Synthetic Genomics

Omschrijving: The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.

MSDS