U heeft gezocht op: Reagentia voor nucleïnezuren

Leverancier: FINNZYMES REAGENTS

Omschrijving: DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.

Leverancier: FINNZYMES REAGENTS

Omschrijving: Met Phusion® Hot Start II High-Fidelity DNA-polymerase kan een groot aantal primers worden gebruikt, inclusief primers met een lage smelttemperatuur. Phusion® Hot Start II High-Fidelity DNA-polymerase is ideaal voor uiteenlopende PCR-toepassingen van standaard PCR-werkzaamheden tot zeer veeleisende toepassingen zoals klonen en PCR met hoge doorvoer. Deze proofreading DNA-polymerase heeft een structuur die vergelijkbaar is met die van Pyrococcus en is voorzien van een uniek dsDNA-bindend domein, waardoor het enzym zeer robuust, snel en nauwkeurig is.

Leverancier: Thermo Fisher Scientific

Omschrijving: The Maxima™ SYBR® Green/Fluorescein qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. It contains SYBR® Green I dye and is supplemented with fluorescein passive reference dye. Fluorescein is included into the master mix to serve as an internal reference for normalisation
of the SYBR Green I fluorescent signal between different wells to compensate for any instrument or pipetting variation. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG)*. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. The use of Maxima™ SYBR® Green/Fluorescein qPCR Master Mix in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

MSDS

Leverancier: Thermo Fisher Scientific

Omschrijving: GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

MSDS

Leverancier: Thermo Fisher Scientific

Omschrijving: Maxima™ Reverse Transcriptase is an advanced enzyme derived by <i>in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Catalogus nummer: (AATB17084)

Leverancier: AAT BIOQUEST

Omschrijving: Sanger method is one of the most reliable and earliest DNA sequencing methods.

UOM: 1 * 1 EA

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Leverancier: AAT BIOQUEST

Omschrijving: 3'-O-Azidomethyl-dATP is one of the four essential dNTPs (A, C, G, T) that can be used for the DNA sequencing by synthesis (SBS).

New Product

Catalogus nummer: (AATB17042)

Leverancier: AAT BIOQUEST

Omschrijving: This iFluor® 555-PEG12-dUTP conjugate has the same spectral properties to ChromaTide™ Alexa Fluor 555-dUTP.

UOM: 1 * 1 EA

New Product Sale

Catalogus nummer: (ICNA117022110)

Leverancier: MP Biomedicals

Omschrijving: MPure™ Forensic DNA Extraction Kit is used with the MPure-12™ aNAP system for the extraction and purification of genomic DNA from forensic samples.

UOM: 1 * 1 Kit

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Catalogus nummer: (M1386-01)

Leverancier: OMEGA BIO-TEK

Omschrijving: Mag-Bind® RXNPure Plus-kit biedt een snelle, betrouwbare isolatie van DNA uit PCR en enzymatische reacties met een hoog terugwinningspercentage. Het systeem combineert gepatenteerde chemische samenstellingen met de omkeerbare nucleïnezuur-bindingeigenschappen van magnetische parels die PCR-amplicons met 100 bp en groter selectief binden en overtollige nucleotiden, primers en kleine, niet-doelgerichte amplificatieproducten, zoals primerdimers, elimineren.

UOM: 1 * 50 mL

MSDS Certificaten Sale

Leverancier: FINNZYMES REAGENTS

Omschrijving: Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Leverancier: FINNZYMES REAGENTS

Omschrijving: Phusion® Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion® Hot Start II DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® Hot Start II DNA polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Catalogus nummer: (ICNA117022400)

Leverancier: MP Biomedicals

Omschrijving: MPure™ Tissue DNA Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for extraction and purification of genomic DNA from a variety of animal tissues, swab samples and blood stains. Nucleic acids extracted and purified from MPure™ Tissue DNA Extraction Kits can be used in a number of downstream applications including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP and Southern Blot Analysis.

UOM: 1 * 1 Kit

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Catalogus nummer: (ICNA117022600)

Leverancier: MP Biomedicals

Omschrijving: MPure™ Bacterial DNA Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for extraction and purification of genomic DNA from both gram-positive and gram-negative bacteria. Nucleic acids extracted and purified from MPure™ Bacterial DNA Extraction Kit can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP and Southern Blot Analysis.

UOM: 1 * 1 Kit

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Leverancier: Thermo Fisher Scientific

Omschrijving: RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.

Catalogus nummer: (K1441)

Leverancier: Thermo Fisher Scientific

Omschrijving: 5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression. The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyse DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected. The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.

UOM: 1 * 1 Kit

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