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Leverancier: MP Biomedicals

Omschrijving: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Leverancier: Thermo Fisher Scientific

Omschrijving: N-Carbamoyl-DL-aspartic acid 98%

MSDS Certificaten

Leverancier: Thermo Fisher Scientific

Omschrijving: 1-Butyl-4-methylpyridiniumhexafluorophosphate ≥99%

MSDS

Catalogus nummer: (BOSSBS-8287R-A555)

Leverancier: Bioss

Omschrijving: Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyzes the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate.Tissue specificity:Liver and kidney.Involvement in disease:Defects in DPYS are the cause of dihydropyrimidinase deficiency (DHPD). DHPD is an autosomal recessive disorder characterized by dihydropyrimidinuria and associated with a variable clinical phenotype: epileptic or convulsive attacks, dysmorphic features and severe developmental delay, and congenital microvillous atrophy.

UOM: 1 * 100 µl

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Leverancier: TCI

Omschrijving: 1-Butyl-4-methylpyridiniumhexafluorophosphate ≥97.0% (by titrimetric analysis)

Leverancier: Thermo Fisher Scientific

Omschrijving: 1-Butyl-4-methylpyridiniumtetrafluoroborate ≥99%

MSDS Certificaten

Catalogus nummer: (ANTIA73754-96)

Leverancier: ANTIBODIES.COM

Omschrijving: Human Carbamylated Albumin ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the <i>in vitro</i> quantitative determination of human Carbamylated Albumin in serum, plasma, tissue homogenates, and other biological fluids.

UOM: 1 * 1 EA

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Leverancier: Thermo Fisher Scientific

Omschrijving: Pyridostigmine bromide 98%

Leverancier: Thermo Fisher Scientific

Omschrijving: 1-Butyl-3-methylpyridiniumtetrafluoroborate ≥99%

MSDS Certificaten

Leverancier: TCI

Omschrijving: Pyridostigmine bromide ≥98.0% (by HPLC, titration analysis)

Leverancier: TCI

Omschrijving: 1-Butyl-4-methylpyridiniumtetrafluoroborate ≥98.0% (by HPLC)

Catalogus nummer: (AATB22030)

Leverancier: AAT BIOQUEST

Omschrijving: DiA is a lipophilic fluorescent stain for labeling membranes and other hydrophobic structures.

UOM: 1 * 25 mg

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Catalogus nummer: (MAYBJFD03653.1)

Leverancier: Thermo Scientific

Omschrijving: 2-Fluoro-1-methylpyridinium-p-toluenesulphonate

UOM: 1 * 1 g

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 This is a MarketSource item. Additional charges may apply

Catalogus nummer: (PRSI29-755)

Leverancier: ProSci Inc.

Omschrijving: UPB1 is a protein that belongs to the CN hydrolase family. Beta-ureidopropionase catalyzes the last step in the pyrimidine degradation pathway. The pyrimidine bases uracil and thymine are degraded via the consecutive action of dihydropyrimidine dehydrogenase (DHPDH), dihydropyrimidinase (DHP) and beta-ureidopropionase (UP) to beta-alanine and beta-aminoisobutyric acid, respectively. UP deficiencies are associated with N-carbamyl-beta-amino aciduria and may lead to abnormalities in neurological activityThis gene encodes a protein that belongs to the CN hydrolase family. Beta-ureidopropionase catalyzes the last step in the pyrimidine degradation pathway. The pyrimidine bases uracil and thymine are degraded via the consecutive action of dihydropyrimidine dehydrogenase (DHPDH), dihydropyrimidinase (DHP) and beta-ureidopropionase (UP) to beta-alanine and beta-aminoisobutyric acid, respectively. UP deficiencies are associated with N-carbamyl-beta-amino aciduria and may lead to abnormalities in neurological activity.

UOM: 1 * 1 EA

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Catalogus nummer: (PRSI91-190)

Leverancier: ProSci Inc.

Omschrijving: beta -Ureidopropionase is a cytoplasmic protein which belongs to the CN hydrolase family of BUP subfamily. beta -Ureidopropionase binds one zinc ion per subunit, catalyzes the last step in the pyrimidine degradation pathway. beta -Ureidopropionase can convert N-carbamyl-beta-aminoisobutyric acid and N-carbamyl-beta-alanine to beta-aminoisobutyric acid and beta-alanine, ammonia and carbon dioxide, respectively. The pyrimidine bases uracil and thymine are degraded via the consecutive action of dihydropyrimidine dehydrogenase (DHPDH), dihydropyrimidinase (DHP) and beta-ureidopropionase (UP) to beta-alanine and beta aminoisobutyric acid, respectively. Defects in beta -Ureidopropionase are the cause of beta -Ureidopropionase deficiency that is characterized by muscular hypotonia, dystonic movements, scoliosis, microcephaly and severe developmental delay.

UOM: 1 * 1 EA

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Catalogus nummer: (786-501)

Leverancier: G-Biosciences

Omschrijving: A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.

UOM: 1 * 50 mL

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