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Omschrijving: Isolate DNA from molluscs and insects using spin columns.

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Omschrijving: Hi-Bind® DNA mini columns are silica glass fibre columns that can bind up to 100 μg of genomic DNA or 35 μg of plasmid DNA per prep. Columns feature an attached lid and a vacuum luer for processing with vacuum manifolds and by centrifugation.

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Omschrijving: Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Omschrijving: E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, <i>E.coli </i>strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

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Omschrijving: The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 30 µg of high-quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA purification is simplified with HiBind® Mini Column technology into three quick steps: Bind, wash, and elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, and manual/automated fluorescent DNA sequencing. E.Z.N.A.® Plasmid Mini Kit I is available in either V-spin or Q-spin column format. V-spin column formats have an attached lid while Q-spin columns do not. Both versions can be used for centrifugation or vacuum protocols.

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Omschrijving: The E.Z.N.A.® Plasmid Maxi Kit is the maxi scale system to deliver high-quality plasmid DNA using the spin column format, without costly accessories. The HiBind® DNA Maxi column facilitates the binding, washing, and elution steps, thus enabling multiple samples to be simultaneously processed. Although yields vary according to plasmid copy number, <i>E.coli</i> strain, and conditions of growth, 50 to 200 ml of overnight culture in LB medium typically produces 0,5 to 1 mg of high copy number plasmid DNA. Up to 500 ml of culture may be processed when working with low copy number plasmid. The product is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, transfection of mammalian cells, transcription<i> invitro</i>, and other applications.

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Omschrijving: E.Z.N.A.® Fastfilter®-plasmidekits zorgen voor een snelle zuivering van plasmide-DNA door middel van lysaatklaringsfilterspuiten, terwijl E-Z 96® Fastfilter-plasmidekits gebruikmaken van lysaatklaringsplaten in een 96-wells indeling. Met Fastfilter®-kits is plasmide-isolatie op midi- en maxischaal mogelijk in minder dan 40 minuten.

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Omschrijving: E.Z.N.A.® Plant RNA kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. This kit provides a homogeniser column for filtration and homogenisation of viscous plant cell lysate by centrifugation in combination with the HiBind® RNA spin column for RNA purification. All the contaminants including polysaccharides and phenolic compounds are effectively removed. Purified RNA can be used for most downstream applications such as RT-PCR, northern blot analysis, differential display, and poly(A)+ RNA selection.

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Catalogus nummer: (B6399-5-48PF)

Leverancier: OMEGA BIO-TEK

Omschrijving: Pre-scripted solution for purification of high-quality DNA from blood, saliva, cultured cells, or fresh or frozen tissue.

UOM: 1 * 48 ST

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Omschrijving: The E.Z.N.A.® DNA/RNA isolation kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenised in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, maximum yield of DNA and RNA can be purified from the entire sample.

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Omschrijving: MicroElute® Clean Up system, designed for rapid DNA clean up.

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Catalogus nummer: (CSPL-1000)

Leverancier: OMEGA BIO-TEK

Omschrijving: Component of the Mag-Bind® plant DNA 96 plus kit.

UOM: 1 * 1 ST

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Omschrijving: De E.Z.N.A.® MicroElute Total RNA-kit biedt een snelle en eenvoudige methode voor het isoleren van maximaal 50 µg totaal RNA uit kleine hoeveelheden gekweekte eucaryotische celweefsels, zoals met laser ontlede monsters (LDS) of fijnenaaldaspiraten (FNA). Normaal gesproken kunnen er tot 5×10⁵ eucaryotische cellen of 5 mg weefsel worden gebruikt in één experiment, afhankelijk van het gebruikte weefseltype. Met deze set kunnen binnen 30 minuten één of meerdere monsters worden verwerkt. Fenol-/chloroformextracties en tijdrovende stappen zoals ultracentrifugering van CsCl-gradiënten en bezinking met isopropanol of LiCl zijn niet nodig. Gezuiverd RNA kan worden geëlueerd met 10 tot 15 µl nucleasevrij water. Gezuiverd RNA is klaar voor de meeste vervolgtoepassingen zoals RT-PCR, northern blotting, poly A+-zuivering, nucleasebescherming en<i> in-vitro</i> omzetting.

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Omschrijving: Modified from the E.Z.N.A.® Plant RNA kit, the E.Z.N.A.® Fungal RNA kit uses an improved buffer system that is more efficient for fungal samples. This kit provides convenient and rapid methods for the isolation of total RNA from a variety of fungal species. Rather, the method involves a simple and rapid precipitation step for removal of much of the polysaccharides and phenolic compounds commonly found in fungal tissues. In combination with HiBind® RNA spin columns, this method permits purification of high quality RNA from as much as 200 mg tissue. The system is also efficient enough to purify total RNA from as little as 10 mg of tissue or 100 cells. Purified RNA can be used for many downstream applications such as RT-PCR, northern blotting, Poly(A)+ mRNA purification, and differential display.

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Leverancier: OMEGA BIO-TEK

Omschrijving: Mag-Bind® Viral DNA/RNA kit is designed for the rapid and reliable isolation of viral RNA and viral DNA from whole blood, serum, plasma, saliva and other body fluids. The Mag-Bind® magnetic beads technology enables purification of high quality nucleic acids that is free of proteins, nucleases, and other impurities. In addition to easily being adapted with automated systems, this procedure can also be scaled up or down, allowing for the purification from various amounts of starting materials. The purified nucleic acid is ready for direct use in downstream applications, such as amplification or other enzymatic reactions.

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Leverancier: OMEGA BIO-TEK

Omschrijving: E-Z® 96 DNA plates are silica glass fibre plates can bind up to 50 μg of genomic DNA or 20 μg of plasmid DNA per prep. Each well has a capacity of 1 ml and the plates are semi skirted. E-Z 96 DNA plates can be processed in vacuum manifolds and/or by centrifugation.