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2,5-Dichloro-4-nitro-3-thiophenesulphonyl chloride

Leverancier: Apollo Scientific

Omschrijving: 2,5-Dichloro-4-nitro-3-thiophenesulphonyl chloride

Nitro blue tetrazolium chloride (NBT) ≥90% (by HPLC), Sigma-Aldrich®

Leverancier: SIGMA ALDRICH MICROSCOPY

Omschrijving: Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridised to its target.